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Fig. 4 | Cancer Cell International

Fig. 4

From: Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma

Fig. 4

Location and sequence homology of the potential Gli1 binding sites (numbers 1–3) in relation to the structure of the MGMT gene. Blank frames represent the five exons and their length (a). The CHIP assay showed only the primers used for the Homo MGMT2 produced the DNA band in the anti-Gli1 lane. ChIP-qPCR also confirmed the result. Human RegIV promoter here served as a positive control for Gli1 binding site (b). Gli1 and MGMT expression were inhibited 72 h after treated by 5 μM cyclopamine in U87 GSP cells measured by immunofluorescence staining and western blot (c). Green (Gli1); red (MGMT); blue (DAPI)

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