Fig. 4
From: Study of the tumor microenvironment during breast cancer progression
The measurement of regulatory T cells (Treg) major markers. RNA was extracted from different stages of breast cancer and control tissue. As main markers of Treg cells FOXP3 (a transcription factor which is mainly related to Treg cell subsets), TGF-β and IL-10 (Treg cell cytokines), CCL22 (a chemokine ligand which plays role in Treg cell migration) and CTLA-4 (an inhibitory receptor on Treg cells) were analyzed by qPCR. The level expression of above genes was determined a in the breast cancer and normal biopsies b in the different stages of tumor and normal breast tissues