Table 2 List of primers
From: Study of the tumor microenvironment during breast cancer progression
No. | Gene code | Forward | Melting point of amplicon |
|---|---|---|---|
Reverse | |||
1 | T.bet | 5′CCAACAATGTGACCCAGATGATT3′ | 85 |
5′TATGCGTGTTGGAAGCGTTG3′ | |||
2 | IL-12p40 | 5′GCCCAGAGCAAGATGTGTCA3′ | 82 |
5′GGGCATCCGGATACCAATC3′ | |||
3 | IFN-γ | 5′TCAGCTCTGCATCGTTTTGG 3′ | 79 |
5′GTTCCATTATCCGCTACATCTGAA3′ | |||
4 | GATA3 | 5′CCCTACTACGGAAACTCGGTCA3′ | 88 |
5′GTAGGGATCCATGAAGCAGAGG3′ | |||
5 | IL-4 | 5′CAAGCAGCTGATCCGATTCC3′ | 81 |
5′TTCTCTCTCATGATCGTCTTTAGCC3′ | |||
6 | IL-5 | 5′CCTGTTCCTGTACATAAAAATCACCA3′ | 80 |
5′TTGAATAGTCTTTCCACAGTACCCC3′ | |||
7 | RORC | 5′ACAGCACCGAGCCTCACG3′ | 85 |
5′CAGACGACTTGTCCCCACAGA3′ | |||
8 | IL-17 | 5′TTGATTGGAAGAAACAACGATGACT3′ | 81 |
5′TGGATTTCGTGGGATTGTGAT3′ | |||
9 | CCL22 | 5′TGCCGTGATTACGTCCGTTA3′ | 87 |
5′CGGCACAGATCTCCTTATCCC3′ | |||
10 | FOXP3 | 5′ACAGCACATTCCCAGAGTTCCT3′ | 82 |
5′GATGAGCGTGGCGTAGGTG3′ | |||
11 | CTLA4 | 5′TGGATCCTTGCAGCAGTTAGTTC3′ | 79 |
5′CATTTTCACATAGACCCCTGTTGTA3′ | |||
12 | IL-13 | 5′AGGTCTCAGCTGGGCAGTTTT3′ | 80 |
5′TAATGATGCTTTCGAAGTTTCAGTTG3′ | |||
13 | STAT3 | 5′CTCAAGAGTCAAGGAGACATGCA3′ | 85 |
5′CTCACTCACGATGCTTCTCCG3′ | |||
14 | FASL | 5′CTCCGAGAGTCTACCAGCCAGAT3′ | 84 |
5′CATGGACCTTGAGTTGGACTTG3′ | |||
15 | CCR7 | 5′GTGGTGGCTCTCCTTGTCATTT3′ | 84 |
5′ATGATAGGGAGGAACCAGGCTT3′ | |||
16 | IL-6 | 5′CCTGAGAAAGGAGACATGTAACAAGAG3′ | 81 |
5′GCAAGTCTCCTCATTGAATCCAG3′ | |||
17 | VEGF | 5′AGGAGGAGGGCAGAATCATCA3′ | 81 |
5′CTCGATTGGATGGCAGTAGCT3′ | |||
18 | IL-10 | 5′GTGATGCCCCAAGCTGAGA3′ | 86 |
5′CACGGCCTTGCTCTTGTTTT3′ | |||
19 | TGF-β | 5′CCTGGACACCAACTATTGCTTCA3′ | 83 |
5′TGCGGAAGTCAATGTACAGCTG3′ | |||
20 | IDO1 | 5′CTCTGCCAAATCCACAGGAAA3′ | 79 |
5′TCTCAACTCTTTCTCGAAGCTGG3′ | |||
21 | SOCS1 | 5′CCCTGGTTGTTGTAGCAGCTTAA3′ | 80 |
5′GGTTTGTGCAAAGATACTGGGTATATG3′ | |||
22 | TNF-α | 5′CCCAGGGACCTCTCTCTAATCA3′ | 84 |
5′ATGGGCTACAGGCTTGTCACTC3′ | |||
23 | ACTB | 5′CAGCAGATGTGGATCAGCAAG3′ | 83 |
5′GCATTTGCGGTGGACGAT3′ | |||
24 | TFRC | 5′ACCGGCACCATCAAGCT3′ | 80 |
5′TGATCACGCCAGACTTTGC3′ |