Fig. 3

Effects of decreased CARP expression on PEM-resistance. a H28-PEM cells were transfected with si-RNA for CARP (three kinds, s502326, s502327 and s502328) at different concentrations and CARP expression levels were examined with Western blot analysis 48 h after the transfection. Tubulin-α was used as a loading control. b Cell viability of H28-PEM cells transfected with si-RNA for CARP (10 nM) or control si-RNA (10 nM) and then treated with PEM for 72 h. The cell viability was measured with the WST assay. SE bars are shown (n = 3)