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Fig. 4 | Cancer Cell International

Fig. 4

From: Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells

Fig. 4

Effects of decreased IGFBP3 expression on PEM-resistance. a–c si-RNA for IGFBP3 (20 nM) (s7227, s7228 and s7229) or different amounts of si-RNA for IGFBP3 (3, 10 and 20 nM), and control si-RNA (si-Cont) were transfected into H226-PEM cells, and suppression of IGFBP3 expression was confirmed by RT-PCR (b) and Western blot analysis (a, c) 48 h after the transfection. Tubulin-α was used as a loading control. Dotted arrow indicates a non-specific signal and arrows shows authentic IGFBP3 signals. d Cell viability of H226-PEN cells transfected with si-RNA for IGFBP3 (3 nM) or control si-RNA (3 nM) and then treated with PEM for 72 h. The cell viability was measured with the WST assay. SE bars are shown (n = 3)

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