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Table 2 Production of IGFBP3 from mesothelioma cells

From: Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells

Source

Cells

Amounts (ng per 1 × 106 cells)

(Average ± SE)

Supernatants

NCI-H28

24.01 ± 0.09

 

H28-PEM

6.31 ± 0.03

 

NCI-H226

100.57 ± 0.18

 

H226-PEM

131.10 ± 0.61

Cell lysate

NCI-H28

3.01 ± 0.02

 

H28-PEM

1.35 ± 0.01

 

NCI-H226

9.56 ± 0.13

 

H226-PEM

23.26 ± 0.37

  1. IGFBP3 amounts from supernatants of 2 days-culture or cell lysate were measured with ELISA
  2. The amounts were calculated with a standard IGFBP3 protein and SEs are also shown (n = 3)