Fig. 3

Expression of EPO and EPOR in HepG2 cells. a Expression of EPO and EPOR mRNA in HepG2 cells determined by regular RT-PCR and normalized to GAPDH mRNA in the same sample. b The histogram displays the ratio between the average level of target mRNA and internal control. c Expression of EPO and EPOR mRNA in HepG2 cells determined by real-time PCR. GAPDH was served as the housekeeping control gene. d Expression of EPO and EPOR protein in HepG2 cells. Total cell lysates were subjected to immunoblotting with specific antibody. β-actin serves as loading control. e the relative densities of EPO and EPOR. Results are representative of three independent experiments. H0, H24, H48 and H72 indicated cells cultured under hypoxia for 0, 24, 48 and 72 h, respectively. *p < 0.05 vs control, **p < 0.01 vs control and ***p < 0.001 vs control. Student’s t test is indicated