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Fig. 5 | Cancer Cell International

Fig. 5

From: A novel Notch1 missense mutation (C1133Y) in the Abruptex domain exhibits enhanced proliferation and invasion in oral squamous cell carcinoma

Fig. 5

Notch1 C1133Y mutation activates the EGFR-PI3K/AKT signaling pathway. a The efficiency of Notch1 transfection was verified in HN6, CAL27 and HN13 cells. EGFR-PI3K/AKT signaling activities were evaluated by western blot analysis. Except that EGFR and AKT levels remained unchanged, wild-type Notch1 decreased the expression levels of p-EGFR, p-Stat5, p-Shc, and p-Gab1, while the Notch1C1133Y mutation reversed the trend in all the tested cell lines. Notch1C1133Y mutation increased p-AKT and PI3K levels in all the tested cell lines, though the p-AKT and PI3K expressions were down-regulated in HN6 or up-regulated in CAL27 and HN13 induced by wild-type Notch1 transfection. b The grey-scale analysis of the alteration of EGFR pathway in panel A

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