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Fig. 1 | Cancer Cell International

Fig. 1

From: MicroRNA 200c-3p regulates autophagy via upregulation of endoplasmic reticulum stress in PC-3 cells

Fig. 1

Ectopic expression of microRNA (miRNA)-200c-3p enhanced the levels of endoplasmic reticulum (ER) stress-related proteins. PC-3 cells were transfected with miR-135a, miR-1290, miR-200c-3p, miR-374b, miR-3195, or control mimics (200 nM). a Western blotting was used to determine the expression of protein kinase RNA-like ER kinase (PERK), inositol requiring protein-1 (IRE1α), 78 kDa glucose-regulated protein (GRP78), activating transcription factor-6 (ATF6), C/EBP homologous protein (CHOP), and β-actin. b Quantitative real-time polymerase chain reaction (RT-qPCR) was performed to determine the mRNA expression levels of ATF6, elF2α, CHOP, and PERK. Levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used for normalization. 1, control; 2, miR-135a; 3, miR-1290; 4, miR-200c-3p; 5, miR-374b; and 6, miR-3195. Data are presented as the mean ± standard error of the mean (SEM) of triplicate samples. *p < 0.05

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