Fig. 2

Effect of SHP-2 on the apoptosis induced by Oxaliplatin and 5-FU through autophagy. Cleaved caspases and cleaved PARP expression were detected by western blot in Hela cells (a), and C33A cells (b). Cells were transfected SHP-2 shRNA1 or pCMV-SHP2-HA plasmid and treated Oxaliplatin (100 μM) or 5-FU (800 μM). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 compared with Oxaliplatin group. ^#P < 0.05, ^##P < 0.01 compared with 5-FU group. Intracellular ROS levels was measured by flow cytometry in Hela cells (c) and C33A cells (d). LC3 expression were detected by western blot in Hela cells (e) and C33A cells (f). Data are presented as mean ± SD. *P < 0.05, **P < 0.01