Fig. 4

Effect of SHP-2 on autophagy activation induced by CCCP in Hela cells. a, b LC3 expression were detected by western blot. Hela cells were transfected SHP-2 shRNA1 and treated CCCP (10 μM) for different time. Data are presented as mean ± SD. **P < 0.01. Induction of GFP + dots in Hela cells expressing GFP-LC3. Cells were transfected SHP-2 shRNA1 and treated CCCP (10 μM). Images of individual cells c and quantified of GFP-LC3 + dots (d). Data are presented as mean ± SD. **P < 0.01