Fig. 6

Representative of flow cytometric analysis of apoptosis with Annexin V and 7-AAD (a, b). The percentages of apoptotic cells were quantified (c, d). down regulation of GPR137 promote apoptosis in K562 (a, c) and HL60 (B, D) cells. There was a significant difference between cells transduced with control lentiviral vectors and those with lentiviral vectors expressing GPR137 (P < 0.001). Representative Western blot of BCL-1 and Caspase 3 expression in K562 (e) and HL60 (f). Lane 1 was cells transduced with control lentiviral vectors and lane 2 was cells transduced with lentiviral vectors expressing GPR137. The expression of BCL-2 was reduced and the expression of Caspase-3 increased in K562 (e) and HL60 (f) cellsCon: cells without infection; Lv-shCon: cells infected with the Lv-shCon; Lv-shGPR137: cells infected with the LvshGPR137; 1: Lv-shCon, 2: Lv-shGPR137. *P < 0.05, **P < 0.01, ***P < 0.001, compared with Con or Lv-shCon cell line