Fig. 1

miR-10b promotes EMT in HCC cells. a In MHCC-97L cells transfected with miR-10b overexpression plasmid and in MHCC-97H cells transfected with anti-miR-10b, morphological changes were observed under a light microscope. b Expression of E-cadherin and Vimentin in MHCC-97L cells with miR-10b overexpression were examined by immunofluorescence. Fluorescence was observed by confocal laser-scanning microscopy. Scale bars, 20 μm. c, d The E-cadherin, Vimentin protein and mRNA levels were detected by western blot and real-time RT-PCR after overexpression of miR-10b in MHCC-97L cells. *P < 0.05