Fig. 5

miR-10b promotes EMT in HCC cells via upregulation of KLF11. a KLF11, E-cadherin and Vimentin expression in HCC cells treated with miR-10b or rescued with si-KLF11 by western blotting. b Real-time RT-PCR analyses of KLF11, E-cadherin and Vimentin mRNA levels as above were transfected. c The expression levels of KLF11, E-cadherin and Vimentin were examined in HCC cells treated with miR-10b or si-KLF11 by immunofluorescence. Fluorescence was observed by confocal laser-scanning microscopy. d Morphological changes were observed under a light microscope. *P < 0.05