Fig. 1

Effect of the mutation sites on p53 expression and transcriptional activity. According to the mutation analysis, five tumors were cultured in 4.5 g/L DMEM medium (b), with the 3 ovarian cancer cell lines, including IGROV-1, SK-OV-3 and OVCAR-3 (a). P53-null SK-OV-3 cells were transfected with four plasmids for 48 h using Attractene transfection reagent (c, d). RNA was extracted from all the cells listed above, and the quantification of the expression of p53, p21 and MDM2 was performed by qPCR using the SYBR green mix. Each value represents the mean of three assays. The control was set to 1, and the values were compared to the control. The results are displayed as the mean ± SD. *P < 0.05 versus the control, and **P < 0.01 versus the control