Fig. 4

Effect of the different domain mutations on glucose consumption and lactate production. After a 48 h incubation in 4.5 g/L DMEM cell culture medium, the supernatants of the IGROV-1, SK-OV-3, and OVCAR-3 cells (a), the transfected cells (c) and the tumor cells (b) were collected. Glucose consumption levels were measured by the glucose oxidase test, while lactate production was assessed by PAP lactate. The values are presented as the mean ± SD of more than three experiments for each cell type and condition. *P < 0.05 versus the control, and **P < 0.01 versus the control