Fig. 3
From: Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC)
Downregulation of PIM1 induces ACC-M cell cycle arrest in G0/G1 phase. ACC-M cells transfected with the indicated shRNA-PIM1 or with scrambled control duplex (shRNA-NC) were cultured for 48Â h and the use of DNA dye PI generates characteristic cellular DNA content profiles. a The PIM1-shRNA transfected ACC-M cells caused inhibition of the G1 stage, histograms represent the percentage of ACC-M cells in G1, S, and G2 phases of the cell cycle 48Â h after shRNA-transfection. b Table representing percentages of cells in G0/G1, S and G2/M phases of the cell cycle after indicated transfection (treatment). Blank: non-transfected normal cell sample; vector control: negative control group; PIM1-shRNA-3 and PIM1-shRNA-4 represent PIM1 knockdown