Fig. 4From: Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC)PIM1 altered the cellular localization of p21 and RUNX3. The localization of p21 and RUNX3 was visualized by immunostaining with anti-p21 and anti-RUNX3 antibodies. When shRNA-NC was transfected into ACC-M cells, p21 and RUNX3 were localized in the cytoplasm. However, when PIM1-shRNA-4 was transfected, p21 and RUNX3 were localized in the nucleus. a The PIM1 protein is expressed in ACC-M cells. b The p21 protein is predominantly cytoplasmic in control ACC-M cells, while p21 is almost exclusively localized to the nucleus 48 h after PIM1 knockdown. c Similarly, RUNX3 protein is nuclear and diffuse cytoplasmic in control ACC-M cells, but almost restricted to the nucleus upon PIM1 knockdown. Blank: non-transfected normal cell sample; vector control: negative control transfection of non-targeting shRNA; PIM1-shRNA-4 is for PIM1 knockdown. All images are captured at ×400Back to article page