Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: Loss of PIM1 correlates with progression and prognosis of salivary adenoid cystic carcinoma (SACC)

Fig. 4

PIM1 altered the cellular localization of p21 and RUNX3. The localization of p21 and RUNX3 was visualized by immunostaining with anti-p21 and anti-RUNX3 antibodies. When shRNA-NC was transfected into ACC-M cells, p21 and RUNX3 were localized in the cytoplasm. However, when PIM1-shRNA-4 was transfected, p21 and RUNX3 were localized in the nucleus. a The PIM1 protein is expressed in ACC-M cells. b The p21 protein is predominantly cytoplasmic in control ACC-M cells, while p21 is almost exclusively localized to the nucleus 48 h after PIM1 knockdown. c Similarly, RUNX3 protein is nuclear and diffuse cytoplasmic in control ACC-M cells, but almost restricted to the nucleus upon PIM1 knockdown. Blank: non-transfected normal cell sample; vector control: negative control transfection of non-targeting shRNA; PIM1-shRNA-4 is for PIM1 knockdown. All images are captured at ×400

Back to article page