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Fig. 1 | Cancer Cell International

Fig. 1

From: The tyrosine kinase inhibitor nilotinib is more efficient than mitotane in decreasing cell viability in spheroids prepared from adrenocortical carcinoma cells

Fig. 1

Morphological characterization of H295R cell spheroid preparations. a Periodic-acid-Schiff staining of glycoproteins; b autofluorescence of collagen fibers; c picrosirius red staining visualized with light microscopy; d picrosirius red staining of collagen type I (orange/red) and collagen type III (green) imaged with polarized light microscopy; e droplets of lipids stained with Oil Red O and fh 3D reconstruction of whole-mount confocal Z-stacks of spheroids, stained with Hoechst and phalloidin imaged with confocal microscopy (Leica TCS SP8); 100 μm

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