Fig. 2

Analysis of viable cells in H295R cell spheroid preparations. a-d Spheroids were stained with Hoechst (blue), annexin V (green) and propidium iodide (PI) (red) after 96 h of culture. Viable (Hoechst-positive); early apoptotic (annexin V-positive and PI-negative); late apoptotic (annexin V-positive and PI-positive) and necrotic (annexin-negative and PI-positive) cells; e–h phase contrast photomicrography of spheroids after 24, 48, 72 and 96 h of culture; 100 μm