Fig. 6
Cell viability analysis of H295R cell line treated with zoledronic acid (ZOL) alone or with mitotane (Mit). MTS assay in monolayer cells after ZOL treatment for 3 days (a) and (b) a combination of ZOL and 10 μM Mit for 72 h; c MTS assay in spheroids after treatment with ZOL or a combination of ZOL and Mit; D) phase-contrast photomicrography of spheroids after 72 h of treatment; e confocal microscopy images of spheroids after 72 h of treatment (100 μm). The results are expressed as the mean ± SEM; ANOVA *p < 0.05; ***p < 0.001; ****p < 0.0001; n = 3