Fig. 7From: The tyrosine kinase inhibitor nilotinib is more efficient than mitotane in decreasing cell viability in spheroids prepared from adrenocortical carcinoma cellsCell viability analysis of H295R cell line treated with imatinib (Ima) alone or with mitotane (Mit). MTS assay in monolayer cells after Ima treatment for 3 days (a) and (b) a combination of Ima and 10 μM Mit for 72 h; c MTS assay in spheroids after treatment with Ima or a combination of Ima and Mit; d phase-contrast photomicrography of spheroids after 72 h of treatment; e Confocal microscopy images of spheroids after 72 h of treatment (100 μm). The results are expressed as the mean ± SEM; ANOVA *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n = 3-4Back to article page