Fig. 8

Cell viability analysis of H295R cell line treated with nilotinib (Nil) alone or with mitotane (Mit). MTS assay in monolayer cells after Nil treatment for 3 days a and b a combination of Nil and 10 μM Mit for 72 h; c MTS assay in spheroids after treatment with Nil or a combination of Nil and Mit; d phase-contrast photomicrography of spheroids after 72 h of treatment; e Confocal microscopy images of spheroids after 72 h of treatment (100 μm). The results are expressed as the mean ± SEM; ANOVA ***p < 0.001; ****p < 0.0001. The results are expressed as the mean ± SEM; n = 3–4