Fig. 2
From: Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D
MCF-12A cells express epithelial and mesenchymal cell markers. E-cadherin is expressed throughout plaque-associated cells and highly localized to cell–cell junctions in the center of epithelial plaques. Localization at cell–cell junctions decreases at plaque edges. Cytokeratins are expressed in all MCF-12A subtypes. Beta-catenin is expressed by epithelial-like MCF-12A colonies with increased localization at their cell–cell junctions and dispersed throughout the cytoplasm of fibroblast-like and spheroid cells. Vimentin is expressed in the cytoplasm of all cells but concentrated in fan-like projections from peripheral cells. P63 is expressed in dividing cells but absent from others. MCF-10A cells used as a control for epithelial marker expression. Scale bar = 100 µm