Fig. 3
From: Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D
MCF-12A cells do not respond to stimulation by estradiol. MCF-12A cells proliferate maximally under all experimental conditions while MCF7 cell proliferation is inhibited in the absence of estradiol and stimulated at increasing concentrations (a). Relative to MCF7 cells, MCF-12A cells do not express estrogen receptor alpha (b). The induction of estrogen responsive genes amphiregulin (AREG) and progesterone receptor (PGR) is not seen in MCF-12A (c) cells while estrogen receptor alpha-positive MCF7 cells show expression following estradiol exposure (d). Note differences in y-axis scales to accommodate variation in expression differences