Table 2 List of antibodies used for immunocytochemistry
From: Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D
Source | Dilution | |
|---|---|---|
Primary antibody | ||
Mouse anti-E-cadherin | Novocastra | 1:75 |
Mouse anti-vimentin | Novocastra | 1:50 |
Mouse anti-pankeratin | Sigma | 1:500 |
Rabbit anti-smooth muscle actin | Abcam | 1:400 |
Mouse anti-p63 | Santa-Cruz | 1:200 |
Mouse anti-beta-catenin | BD | 1:500 |
Secondary antibody | ||
Goat anti-mouse peroxidase | Pierce | 1:1000 |
Goat anti-rabbit peroxidase | Pierce | 1:1000 |