Table 3 Estrogen responsive gene induction assay primer sequences
From: Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D
Gene | Forward primer | Reverse primer |
|---|---|---|
L19 | 5′-TAGTCTGGCTTCAGCTTCCTC-3′ | 5′-TCTGCAACATCCAGCTACCC-3′ |
Estrogen receptor alpha | 5′-TAAATGCTGCCATGTTCCAA-3′ | 5′-CCTGTGAGAGAACAGAAACTGG-3′ |
Amphiregulin | 5′-GTGGTGCTGTCGCTCTTGATACTC-3′ | 5′-TCAAATCCATCAGCACTGTGGTC-3′ |
Progesterone receptors A/B | 5′-GAGGATAGCTCTGAGTCCGAGGA-3′ | 5′-TTTGCCCTTCAGAAGCGG-3′ |