Fig. 2
From: MicroRNA-181c prevents apoptosis by targeting of FAS receptor in Ewing’s sarcoma cells
Inhibition of FAS mRNA expression in SKES1 cells. a Possible binding sites of miR-181c at the 3′UTR of FAS mRNA. Each sequence of miR-181c (Wt) and its mutant (Mut). b, c After actinomycin D administration, the miR-181c and mRNA expression level in the negative control-miR, miR-181c, and miR-181c mutant was evaluated by qRT-PCR. *p < 0.05, **p < 0.01. d Western blot analysis showed an increase in FAS protein levels upon anti-miR-181c and FAS-expression vector treatment. e Densitometric evaluation of FAS protein. *p < 0.05, **p < 0.01. f Western blot showing increase in the expression of FAS protein by the transfection of FAS expression vector in SKES1 cells. g The expression of FAS protein after induction of FAS vector. **p < 0.01