Fig. 3
EGCG and EC induced cell cycle arrest, Bcl2 activity, and ROS production in HepG2 cells. Cells were treated with EGCG and EC for 72 h. Controls were treated with 0.1% DMSO. a Cell cycle arrest was investigated by PI staining and detected by flow cytometry. Representatives of the cell population during cell cycle phases and b quantitative percentages of all cell cycle phases are expressed in the histogram. c Activity of Bcl2 was determined by MUSE Cell Analyzer flow cytometry and percentage calculated for Bcl2 activity compared with 100% of the vehicle control. d ROS production was determined by CM-H2DCFDA fluorescent dye, detected by flow cytometry, and percentages calculated for ROS product compared with 100% of control. Data are expressed as mean ± SD from at least a triplicate of n = 3, *p < 0.05, denoting significant differences compared with the control