Fig. 3

p-EGFR and intergrin β4 expression were assayed by IFA (a) and p-EGFR and p-FAK were assayed by western blot (b). Integrin β4 over expression induced rapid internalization of surface EGFR to the cytoplasm, while integrin β4 knockdown promoted the expression of EGFR. c The interaction between p-EGFR and intergrin β4 was assayed by immunocoprecipitation with anti-p-EGFR antibody. The first two samples were from SGC7901 cells and the second two samples were from clinic gefitinib-sensitive samples. *P < 0.05 vs. SCG7901. ^#P < 0.05 and ^##P < 0.01 vs. SGC7901R