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Fig. 2 | Cancer Cell International

Fig. 2

From: microRNA-338-3p inhibits proliferation, migration, invasion, and EMT in osteosarcoma cells by targeting activator of 90 kDa heat shock protein ATPase homolog 1

Fig. 2

AHSA1 is a direct target of miR-338-3p. a Predicted duplex formation between the 3′UTRs of wild-type or mutant AHSA1 and miR-338-3p. b Luciferase activity of wild-type (Wild 3′UTR) or mutant (Mutant 3′UTR) AHSA1 3′UTR reporters in 293T cells transfected with a miR-338-3p mimic or NC. c qRT-PCR data showing AHSA1 mRNA levels in MG-63 and Saos2 cells transfected with a miR-338-3p mimic or NC. Data were normalized against GAPDH mRNA levels. d Western blotting of AHSA1 in MG-63 and Saos2 cells transfected with a miR-338-3p mimic or NC. Data are expressed as mean ± SD, *P < 0.05

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