Fig. 3

The downregulation of RUNX3 and TGF-β in murine renal tumor xenograft model in vivo. a The xenograft renal tumor metastasis and primary models were established after injection of 1 × 10⁴ corresponding cells in the left foreleg of mice, respectively. The survival days were calculated after each mouse was dead (n = 10, p < 0.01). b Tumor volumes in xenograft models inoculated from renal metastatic tumor cells and primary tumor cells were measured 2 months post inoculation while all the mice were still alive but sick. Values are presented as mean ± SD from at least three independent experiments (**p < 0.01). c RUNX3 and TGF-β expression in metastatic and primary renal cancer models were detected by Western blot. Relative expression values represent mean ± SD from three independent experiments (**p < 0.01). Quantitation by densitometry is also shown