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Fig. 6 | Cancer Cell International

Fig. 6

From: Epithelial to mesenchymal transition induces stem cell like phenotype in renal cell carcinoma cells

Fig. 6

EMT induced cells acquire stem cell-like phenotype: a immunofluorescence show expression of the marker for pluripotency i.e. Oct-4 in CsA (10 µM) treated A498 cells. Magnification ×10. Scale bar—20 µm. b Agarose gel electrophoresis (2%) showing expression of the stemness gene KLF4 in A498 cells with or without the treatment of CsA (8, 10 μM) and β-actin was used as housekeeping gene. c Tumorisphere formation assay: Bright field image showing tumorisphere formation by CsA treated cells grown on non-adherent surface in serum free media. Magnification ×10. d Graph showing the KLF4 expression in control and CsA treated A498 cells (8, 10 μM) done by densitometry

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