Fig. 6From: Autophagy inhibition improves the cytotoxic effects of receptor tyrosine kinase inhibitorsPost-treatment with the SP1 agent. RTKi were used as a single pre-treatment or in combination with Spautin-1 (SP1; 20 μM; added 2 h upon use of RTKi). Changes in LC3, p62, PARP and Caspase-3 protein levels were evaluated by western blot. GAPDH served as a loading control protein. D DMSO, SP1 Spautin-1, Af Afatinib (8 μM), Sor Sorafenib (14 μM)Back to article page