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Fig. 7 | Cancer Cell International

Fig. 7

From: Autophagy inhibition improves the cytotoxic effects of receptor tyrosine kinase inhibitors

Fig. 7

Autophagy activation in Kelly cell line upon treatment with RTKi. a Autophagy activation upon treatment with Afatinib and Sorafenib was validated using immunofluorescence in order to visualize a creation of autophagosomes; ×60 magnification. DMSO treatment was used as control. Green—autophagy vacuoles; Blue—Hoechst, nuclear staining. b Increasing concentrations of Afatinib and Sorafenib were used for the treatment of Kelly cells for 24 h (numbers indicate the concentrations used in μM). Levels of main autophagy and apoptosis related proteins were evaluated by western blot. GAPDH served as a loading control protein. D DMSO. c RTKi were used as a single treatment or in combination with CQ (25 μM). Changes in autophagy and apoptosis related proteins were evaluated by western blot. GAPDH served as a loading control protein. D DMSO, CQ Chloroquine, Af Afatinib (8 μM), Sor Sorafenib (12 μM)

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