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Fig. 3 | Cancer Cell International

Fig. 3

From: MicroRNA-365 promotes lung carcinogenesis by downregulating the USP33/SLIT2/ROBO1 signalling pathway

Fig. 3

USP33 is a downstream target gene of miR-365a-3p and is downregulated by miR-365. a Expression levels of USP33 were detected by western blotting of A549 or SPC-A-1 cells after transfection with agomiR-365, antagomiR-365, or controls. β-tubulin was used as an internal control. Experiments were performed at least three times, and representative bands are shown. b (a) Sequence alignments of miR-365a-3p binding sites in the wild-type and mutant USP33 3ʹ UTRs. The replaced site is underlined. (b) Wild-type USP33 3ʹ UTR or mutant USP33 3ʹ UTR was co-transfected with agomiR-365 or agomiR-NC into A549 or SPC-A-1 cells. After 48 h, relative luciferase activities were measured. *P < 0.05, **P < 0.01, ***P < 0.001. c Expression levels of miR-365a-3p and USP33 were examined by qRT-PCR in 20 paired human lung adenocarcinoma tissues and adjacent normal lung tissues. A correlation was observed between miR-365a-3p and USP33 expression as determined by Spearman correlation analysis (n = 20; R2 = 0.2467, P < 0.05)

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