Fig. 4
From: MicroRNA-365 promotes lung carcinogenesis by downregulating the USP33/SLIT2/ROBO1 signalling pathway
miR-365a-3p promotes lung cancer cell proliferation, migration, and invasion by downregulating USP33. a, b The proliferative ability of A549 or SPC-A-1 cells after overexpression of USP33 by transfection of pcDNA3.1-USP33, miR-365a-3p by transfection with agomiR, or USP33 and miR-365a-3p simultaneously was measured using the a plate clone formation and b EdU assays. pcDNA3.1-vector and agomiR-NC were used as negative controls. c, d The migration and invasion activities of A549 or SPC-A-1 cells transfected with pcDNA3.1-USP33, agomiR-365, or both vs. controls were measured using the c Transwell migration and invasion and d wound healing assays. Left panel, representative images. Right panel, quantitative data. All experiments were performed at least in triplicate and repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001 compared to controls