Fig. 1

CHIP analysis of LNCaP cells treated with the LSD1 inhibitor LSD1-C76. LNCaP cells were incubated in media with charcoal stripped serum (CSS) for 24 h prior to addition of dihydrotestosterone (D, 1 nM) ± LSD1-C76 (C76, 250 μM) for 24 h. CHIP was performed using histone H3 and histone H3 K9 mono-methylated antibodies. Precipitated chromatin was amplified using primers flanking the enhancer region (ARE III) of the PSA gene or actin gene promoter [14]. DMSO dimethyl sulfoxide solvent control