Fig. 2

Chemical inhibition of LSD1 reduces AR, PSA and LSD1 expression and induces PARP cleavage. a Western blot analysis of the indicated targets in LNCaP prostate cancer cells, grown in charcoal stripped media for 24 h before exposure to dihydrotestosterone (DHT, 1 nM) or LSD1-C76 (250 μM) for 24 or 48 h as indicated. b–e Quantification of PSA (b), LSD1 (c), cPARP (d) and AR-WT (e) protein levels by densitometry analysis and normalized to β-actin. Western blots are representative of two independent experiments. P values were derived using 2 Way ANOVA multiple comparisons test. cPARP, cleaved PARP; ** and ***P < 0.005 and P < 0.001 respectively