Fig. 2

a Induction of cyclooxygenase (COX)-2 by CD required activation of PKCα in MKN-74 cells. Exponentially growing MKN-74 cells were exposed to control, 10 nM PMA, and CD in 100, 200 and 400 μM respectively for 24 h; then cells were collected and fractionated into membrane (M) and cytosolic (C) extracts. Western blots analyses were performed using COX-2 and PKCα antibody. Induction of COX-2 protein expression was associated with activation of PKCα, as demonstrated by translocation from cytosolic fraction to membrane fraction. b UDCA did not affect PMA- or CD-induced COX-2 protein expression. Exponentially growing MKN-74 cells were exposed to control, 10 nM PMA, 200 μM CD, 200 μM UDCA, 10 nM PMA plus 200 μM UDCA, or 200 μM CD plus 200 μM UDCA for 8 h, then cells were collected and fractionated into membrane (M) and cytosolic (C) extracts. Western blots analyses were performed using COX-2 antibody. UDCA did not affect PMA- or CD-induced COX-2 protein expression