Fig. 6

Hetero-regional expression analysis of sub-areas across consecutive sections for all meningioma tissues. a A diagram depicting consecutive sectioning and immunofluorescence staining for each section. b Bar graphs showing the number of different types of sub-areas for all tumors separated into significantly differential groups (R1, R2, R3) or grades (grade I, grade II/III). c Regions that significantly frequently occurred in grade II/III but never in grade I meningiomas. d Representative immunofluorescence images for consecutive sections for significantly frequently occurring combinations in grade II/III meningiomas. Sections were double stained for Ki67 (red) with Nestin (green), SOX2 (red) with CD133 (green), Vimentin (green) with FZD9 (red), SSEA4 (green) with SOX2 (red), and SSEA4 (green) with Olig2 (red), each with DAPI (blue). Single staining of GFAP (red) and BIIITubulin (red) is also shown. All images were taken at ×20