Fig. 2
Harmine induces NB cell death. a NB cell lines (SKNBE, KELLY, SKNAS, and SKNFI) were treated with 100 μM harmine or left untreated for 24–72 h. Representative micrographs show the effects of 100 μM harmine on cell morphology after 72 h (see Additional file 1: Fig. S1 for micrographs representative of harmine treatments after 24 and 48 h). b IC50 curves representing cell viability measurements using Real-Time Glo (Promega). NB cell lines were continuously exposed to a range of harmine concentrations (0–1 mM) for a total of 72 h, with measurements after 24, 48, and 72 h. Values were normalized to control and represent the average of three independent experiments ± S.D. (n = 3)