Fig. 5
Harmine induces apoptosis, marked by increase in Annexin V-positive NB cells. a NB cell lines (SKNBE, KELLY, SKNAS, and SKNFI) were incubated with 100 μM harmine for 24 h, after which Annexin V presence was analyzed by flow cytometry using the Annexin V Apoptosis Detection Kit APC. The numbers in each quadrant represent the average number of events of three experiments (n = 3). b Bar graph representation showing an increase in the percentage of total apoptotic cells with harmine treatment. Data represent the average of three experiments ± S.D. (n = 3). Asterisk denotes a statistically significant increase in the percentage of apoptotic cells compared to control. The p-values are as follows: SKNBE P = 4.4 × 10−2; KELLY P = 1.3 × 10−3; and SKNAS P = 3.6 × 10−2. The p-value of SKNFI cells was not significant