Fig. 5

CTGF was a direct target of miR-218 and could restore its inhibition effects on CRC progression. a, b miR-218 was able to suppress the mRNA (a) and protein (b) expression of CTGF. c, d miR-218 could inhibit the luciferase reported activity to 50%, demonstrating that it directly targeted CTGF. e CTGF treatment rescued the inhibition effects on EMT associated proteins brought by miR-218 over expression. f CTGF treatment restored the inhibition effects on VEGFA and ANGPT2 brought by miR-218 over expression. N = 3, **P < 0.01, t-test