Fig. 2From: Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrinsFMNL2 shRNA decreases FMNL2 level in HGC-27 cells. a mRNA level of FMNL2 in HGC-27 cells was measured by quantitative real-time PCR after FMNL2 silencing. mRNA level of FMNL2 was normalized to β-actin, and relative mRNA level was calculated using 2−ΔΔCt method. b, c After FMNL2 silencing, protein level of FMNL2 was assessed by western blot with β-actin as internal control. Each experiment was repeated three times. The results were shown as mean ± SD. ***p < 0.001 compared with negative control cellsBack to article page