Fig. 6
From: Integrin β1 regulates the invasion and radioresistance of laryngeal cancer cells by targeting CD147
Effects of MβCD on the expression and location of integrin β1 in laryngeal cancer Hep-2 cells. a The mRNA expression of integrin β1 was analyzed by qPCR. b The protein expression of integrin β1 was analyzed by western blotting. GAPDH was used as an internal control. c Location of integrin β1 in lipid raft fractions. d Cells treated with MβCD were double-stained for GM1 (green) and integrin β1(red). Cell nuclei were stained with DAPI. e Co-immunoprecipitation of integrin β1 and CD147 in Hep-2 cells. f The expression of FAK/cortactin signaling was analyzed by western blotting. #p > 0.05 compared with untreated Hep-2 cells. ** Unspecific band