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Fig. 1 | Cancer Cell International

Fig. 1

From: Interferon-γ and Smac mimetics synergize to induce apoptosis of lung cancer cells in a TNFα-independent manner

Fig. 1

IFNγ and Smac mimetics synergistically induce cell death in the H1975 NSCLC cell line. a, b H1975 NSCLC cells were incubated with 1–25 units of human IFNα or 1–25 ng/ml of IFNγ, IFNλ or TNFα, TRAIL (500 ng/ml), cisplatin (25 µM) or poly(I:C) (500 ng/ml) in the presence or absence of 20 nM AZD5582 for 48 h. cf H1975 cells were incubated with 10 ng/ml IFNγ or 250 ng/ml poly(I:C) plus different doses of AZD5582 (c), SM164 (d), BV6 (e) or Birinapant (f) for 48 h. Cell viabilities (af) were assessed by MTS assay and cell survival rates were calculated by comparison to DMSO-treated control cells and are presented as mean ± SE (n = 3). g H1975 cells were treated with IFNγ (10 ng/ml), poly(I:C) (PIC, 500 ng/ml), IFNγ plus poly(I:C), or transfected with poly(I:C) (tPIC, 100 ng/ml) alone or plus IFNγ in the presence or absence of 20 nM AZD5582 for 48 h. Viable cells were counted with trypan blue by a TC20 automated cell counter (n = 3). h H1975 cells were treated with IFNγ (5 ng/ml) in the presence of DMSO or AZD5582 (20 nM) for 48 h, and the detached cells were collected and counted with trypan blue by a TC20 automated cell counter (n = 3). **p < 0.01; ***p < 0.001 versus DMSO. Results represent the findings of three independent experiments

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