Fig. 3
From: ETS1 is associated with cisplatin resistance through IKKα/NF-κB pathway in cell line MDA-MB-231
DDP drug resistance protein, activation of NF-κB pathway ETS1 mRNA expression is correlated with overall survival. a mRNA expression validation of six predicted drug-resistant genes via qRT-PCR; b 231/wt and 231/DDP cells were cultured in 6-well plates for 48 h. Western blot analysis was performed to measure the protein expression of P38, p-P38, ETS1, IKKα,IKKβ and NF-κB p-65 (nucleus) between 231/wt and 231/DDP cells. c Kaplan–Meier curve shows overall survival differences between relative low-expression ETS1 group and high-expression group (P = 0.0112). d Immmunofluorescence staining localization of NF-κB by antibody and nuclei staining by DAPI between 231/wt and 231/DDP cells