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Fig. 3 | Cancer Cell International

Fig. 3

From: Long noncoding RNA PVT1 modulates hepatocellular carcinoma cell proliferation and apoptosis by recruiting EZH2

Fig. 3

PVT1 could bind to EZH2. a RIP assay indicated that PVT1 levels of EZH2 RIP vs IgG RIP in si-PVT1 group was higher than that in si-NC group. **P < 0.01, compared with si-NC group. b QRT-PCR indicated that EZH2 mRNA expression in si-PVT1 group had no significant difference between that in si-NC group. c RNA pull-down assay indicated that PVT1 bound to EZH2. d Western blot revealed that EZH2 protein expression in si-PVT1 group was lower than that si-NC group. *P < 0.05, compared with si-NC group. e Western blot indicated that after added with 100 μg/mL CHX, si-PVT1 had greater inhibitory effect on EZH2 protein expression with the increase of time. f ELISA assay revealed that EZH2 protein expression in si-PVT1 group was lower than that in si-NC group with the increase of time after added with 100 μg/mL CHX. *P < 0.05, compared with si-NC group

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