Table 1 Primer sequence
Gene | Sequence |
|---|---|
PVT1-F | 5′-ATAGATCCTGCCCTGTTTGC-3′ |
PVT1-R | 5′-CATTTCCTGCTGCCGTTTTC-3 |
GAPDH-F | 5′-GGAGCGAGATCCCTCCAAAAT-3′ |
GAPDH-R | 5′-GGCTGTTGTCATACTTCTCATGG-3′ |
EZH2-F | 5′-TTGTTGGCGGAAGCGTG-3′ |
EZH2-R | 5′-TCCCTAGTCCCGCGCAATGTGC-3 |
pcDNA3.1-PVT1-F | 5′-GGGGTACCCTCCGGGCAGAGCGCGTGTG-3′ |
pcDNA3.1-PVT1-R | 5′-CGGGATCCTAGACACGAGGCCGGCCACGC-3′ |
si-EZH2 | 5′-AUCAGCUCGUCUGAACCUCUU-3′ |
si-NC | 5′- GGGCCAGACTGGGAAGAAA -3′ |
si-MDM2 | 5′-GTGTGTAATAAGGGAGATA-3′ |
si-PVT1 | 5′-CAGCCATCATGATGGTACT-3′ |